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Human element [ hs35 ]


Position: chr16:54,665,329-54,667,258 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: IRX3 - CRNDE

Timepoints:
e11.5

Publications:

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
AATGAAATTGAATCCAAACCTTGTGgaagtcagaacccactggaaaagacctctggtccc
ccctgggacaggccagtgggacaggaagtctgccggctccagttccagccatgggcctcc
gcagaagcaccctgcacacagcctcgcgctgtggcatgaattccaggcgaaggaagggtc
aagatgacgggacaagagcagacggggatcacgaagcccctggatcaaccgaggtcccat
catgaggtgggagaaatgatggatttatatatttaatgaaagggtaactctggaagagta
ataaaaatatggtcaagtaggcaggtatgtttggcgctttttttttttttttttttttaa
atctacactctggagctctcactctggtacaattcttttgttagatcaggtaatattttt
gaaaaacagcattacaaaaggttaaatgtagaacagatgtaagttataaaatacaagctg
atcaaagctgagattttgagacagatatgttagcaaatgatgataaatccgtcccagtag
gactgtaagaagcagacaaatgtaactatgggagagtgaaagtcaagggttgaaactggg
ggaaggggagaaagagtgaaaacaaatgttgaagttaattctggtgaactcacgcagaac
atcaatgaatctatgccgagataggacagtcagaacggacacaatatgctccattttcct
gaaaagattcatcagattcctgggttttgaataatccaattatggattcagatccccaaa
tctcagggatctctctttataccagtttctgactctagatttcaaccttttcaagagaca
gagcctgggttctggcttaaagctttaaaaatcggctttagagtttgggtgtctaaactt
ctcattaaaaagccagataatttatagtcatatcattaaaccgcttcctcagcctaccgt
gataaacttatcattttggggggggtagatttcaacaaaacattatgaagactcctttct
tggaaggcggcagagaaacaaaattaaataaatcaagcctctctgtgtcaataaatgaag
cccatgagaagtaaactatttactgaggcttaagtgaaacagaaaacatttaaagatcaa
gaaaaaaggaggtaattgttcagcctgtcagcaggggaccgctgtgataatttgatctga
actctgctaacaaatgaccgagccgttcttccaactttagtatctgtttatgctcctaca
aattgaaaccccacagctcagctgcacagaacagggcccagtacaatagcacattggccc
gacgattagttcaagctggggatggattagctgtccatcccaggcctccaaaccctccag
aatattttgtcttccctgctgaggtaaaagggtgcagacatatgcaactaataaagaaac
aggcttctatggactggaggttgagtggaaggaatgcagaacaaaactataaaataataa
tagacaaaggggcagtgaggactggggaactcaataccccctctgcttacccaggcagga
ccgtagcacccacatccccgcaagttttcaaaacggaaggggcctggtgcatgtgtgatc
tcccagcacatggttgtgggcctggcgtgccaacgttcagggctgacatgagatcaaaag
cctgataaatgagaggaaagggaatgtttgacccactaaaccatattctacaagaatctt
gggcttttaaagtaataagaagggctataaattacaatgtcttgggtatattctgatgca
catgaatgttactcacatgtaatgtttttagagagattcatgagcaattgaatgcttaag
aatccagtcccctttgacaattctgcatctccagtcagagtccacATTTGGGGAAAAGCA
ACTTATCATC
Primer
(+)aatgaaattgaatccaaaccttgtg
(+)gatgataagttgcttttccccaaat